The SPAD502 Chlorophyll meter is based on different chlorophyll content to determine the chlorophyll content of two different wavelengths of light, which is fast, simple and non-destructive. The measurement result is the relative value of the chlorophyll content in the leaves of plants. Plants have been used in applications. However, the determination of SPAD value and chlorophyll content in leaves of wild white peony and their correlation analysis are rarely reported. This experiment has made a preliminary exploration of this side and aims to provide a new way for the rapid determination of the chlorophyll content of the wild white peony germplasm resources. Determination of SPAD502 values ​​Potted plants were harvested from wild white peony roots, intact leaves with no mechanical damage, no pests and lesions were selected, and the dirt and dust on the surface of the leaves were wiped off and measured with a SPAD502 chlorophyll meter. Leaf blade. The assay sample was used as a sample for chlorophyll content determination.
The determination of chlorophyll content in SPAD502 is a rapid and indirect method. In order to verify its effective feasibility in the determination of chlorophyll content in white peony, correlation analysis between chlorophyll content and SPAD value of 20 white peony varieties can be seen. The correlation between the chlorophyll content and the SPAD value was also different between different varieties of white peony. There was a significant linear correlation between the chlorophyll content and the SPAD value of all white peony varieties. The maximum correlation coefficient can reach Q99, and the lowest correlation coefficient also reaches a significant level. These results indicate that there is a positive correlation between leaf SPAT values ​​and chlorophyll content when using the SPAT values ​​to determine the relative values ​​of chlorophyll content. Therefore, the SPAD value of the leaf of white peony can be used as the nutrient requirement during the growth of white buckwheat. One of the indicators.
The use of SPAD502 chlorophyll meter for the determination of chlorophyll a, chlorophyll b, total chlorophyll content and SPAD values ​​of chlorophyll of different species of white peony found that they were very different, and there was no significant difference among most varieties. According to the correlation analysis between the chlorophyll content and the SPAD value, there is a significant linear correlation between the 20 white grub varieties.
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