Effect of Salt Stress on Seeds of Peacock Grass by Illumination Incubator

The cultivation of peacock grass on the flowerbed is very common, but the research on it is still focused on the cultivation. The research on the germination characteristics of seeds and the seed germination under salt stress is still rare. Therefore, in order to be able to grasp the germination of their seeds more thoroughly, it is necessary to conduct detailed research on them. The effects of different concentrations of salt solution on the germination status of peacockgrass seeds were studied to reveal the mechanism of salt resistance and to explore the response of different concentrations of salt during the germination of peacock grass seeds, aiming at a large number of cultivation applications in production. Peacock grass provides scientific basis. The test instrument must use a light incubator in order to simulate the growth environment.

The experiment was completely randomized. O(CK), 2,4,8,12g/I, 4 concentration gradient NaCI solutions and one control were set up, and 100 seeds and 3 replicates were processed. According to the International Seed Inspection Regulations, double-layer filter paper is used for germination. In a petri dish with a diameter of 9 cm, a 10 cm diameter solution of NaCI is added to the petri dish with a double layer of filter paper (concentrations: O(CK)}2}4}8}12 g/L). After the filter paper is fully moistened, The residual water of the peacock grass seeds after disinfection and soaking in warm soup was sucked with clean absorbent paper, and the seeds were evenly placed in the petri dishes. During the seed placement, there is a certain gap between the species for germination and observation counting. 100 grains per dish, covered with lids, affixed labels, placed in C25 ± 1 °C I, light incubator, germination test.

Observe seed germination every morning and evening, record seed germination status and seed germination counts, and write observation time and observers. The seed germination is based on the criterion that the radicle breaks through the seed coat and the length of the hypocotyl is not less than 1/2 of the length of the seed itself. When the seed germinates at a low level or the 3rd consecutive germination number no longer grows, it is more than the germination test group. When the solution in the petri dish cannot be immersed in the seed itself during the observation period, 2^} drops of distilled water are added with a dropper to prevent the seed from germinating. During the observation process, if there is a phenomenon of seed rotting, the mercury-enriched solution can be used for disinfection and then returned to the original place for observation. The severely rotten seeds should be immediately picked to avoid infecting other seeds and recorded as the number of ungerminated seeds. Finally, the fresh weight of each treated seedling was weighed.

Through the light incubator on the seed germination test of the peacock grass, it can be found that with the increase of the concentration of the salt solution, it first rose and then decreased. When the salt concentration was 29/l 2 , the seed germination rate was slightly higher than that of the control, indicating that the seedling emergence rate of the peacockgrass seed was higher in this concentration range, but when the salt solution concentration was higher than 29/l two, the peacock The germination of grass seeds has been severely inhibited. Although there is still a certain germination rate, the germination rate is low.

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