Chromatography is a separation science. HPLC, like other kinds of chromatograms, is separated according to the structure of the sample. There are many kinds of HPLC. They can often separate substances and the separated component peaks should be detected. Usually, the most commonly used tests are The instrument is a UV/VIS or fluorescence detector, but many substances cannot be detected or are difficult to distinguish from the background so that they cannot be detected. Post-column derivatization techniques are a good solution to this problem. Post-column derivatization systems are also known as Post-column reaction system, the main purpose is to make the original undetectable material into a detectable material, this method is mainly to pass the separated material through the reaction to make it have detectable physical properties. The typical method is through a The reaction gives the substance a colored group or allows the substance to produce fluorescence. In some cases it can increase the detection sensitivity by several orders of magnitude. Most of the solvents have a certain selectivity for a particular type of substance. Thus it can be detected from a complex background. So the biggest role of post-column derivation is to increase sensitivity and selectivity.
The post-column derivatization system is to mix HPLC post-column eluent and derivatization reagent. The mixed solution will complete the reaction in a certain time through the reaction system. If the reaction is slow, the reactor will be heated to increase the reaction rate. Some reactions need to be added. The two solvents, the final mixture entering the detector are generally detected by UV/VIS or fluorescence. A complete post-column derivation system requires high-precision metering pumps, pulse dampers, heated reactors, safety systems, etc. to ensure safety, Reliable data.
Application industry:
Environmental Water Quality Surveillance Feed Industry Nutrition Analysis and Poison and Residue Monitoring
Food Industry Nutrition Analysis and Monitoring of Toxic Substances and Residues Clinical Diagnostic Monitoring and Prenatal Screening
Pharmaceutical chemical product content analysis and residue monitoring.
main feature:
Quick and easy to set up Simply connect an inlet from the column and an outlet into the detector. The installation is completed instantly. The modular design can be selected with single or dual reaction cores. The reaction can be carried out at the same time with a two-step reaction at room temperature. Optional one, two or more derivatization pumps. Reduces fittings and tubing connections to prevent leaks. All fittings are located on the front panel for easy use by the user. Panel display for easy setup of the reactor front panel to display reactions Core set temperature and actual temperature. Derivative pump front panel displays the flow rate, pressure and pressure limit of each reagent. External control pump flow rate and the temperature of the reactor can be controlled by the front panel, or through RS232 external control module. The reactor can be exchanged with only 2 connectors. The new design of the reaction core greatly reduces the peak diffusion.
Post-column derivatization system features
1, fast and easy to set up (PCX-A)
Simply connect an interface from the column, derivatization solution and detector.
2. Special flow path design (PCX-C)
Optional built-in column thermostat and injection valve in reaction system reduce dead volume
Can be switched between conventional applications and post-column derivation systems by moving six-way valves
With various domestic and foreign HPLC pump and detector docking, a wide range of applications.
3, reactor design
The reactor adopts a column spiral design, heating is more uniform and the reaction is more complete.
The reaction pipeline is made of PEEK material, which is more resistant to corrosion.
Built-in 1m, 3m, 5m PEEK tubing with inner diameter of 0.5mm, according to different experiments to choose the length of the pipeline
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