Protein Determination The most common non-Kjeldahl method is the one that can determine the protein content of various crops and foods. It is widely used at home and abroad and has been used as a standard test method. In order to increase work efficiency and accuracy, the Kjeldahl device was replaced by a digester and an automatic Kjeldahl instrument to replace the manual operation. 20 sample tubes can be digested at one time. The instrument automatically completes the dilution with water, adds alkali, and adds Acid, distillation, titration, digester emptying and results calculations are fast and accurate. Therefore, the automatic Kjeldahl nitrogen analyzer has great advantages and application prospects in the determination of crude protein content in fields such as agriculture, food, chemical industry, medicine and health.
Select a representative seed to pick clean and shell, sample and grind, pass 0.42mm sieve. Weigh 0.5g sample 2 copies, accurate to 0.0001g, according to the national standard GB5497-1985 determine the moisture content of the sample. Open the digester and preheat it so that the temperature reaches 420 °C for digestion. Place the test crop sample in a 250-mL digestive canal (be careful not to adhere to the wall to prevent incomplete digestion). Except for soybean crop plus 1 g sample, all other crops are 0.5-1.0 g. After adding the digestion tablets 5g, then add 12 ~ 15mL concentrated sulfuric acid, gently shaking the digestive tube, the sample was wet with sulfuric acid. The digestion tube was placed on a digester preheated to 420°C and the suction pump was turned on to a maximum. After 5 minutes, a small suction pump was turned off until the acid mist just filled the exhaust hood and a condensation ring was formed in the test tube. About 90 min sample digestion to a clear blue-green liquid, remove and cool to room temperature.
Put the digestive tube into the automatic Kjeldahl nitrogen analyzer, close the safety gate, set the list, and start loading the sample after registration. The instrument is automatically distilled, titrated, and the results calculated and cleaned. Instead of using 0.1g of sucrose as a blank sample, the volume of the spent acid standard solution must not exceed 0.2mL.
The laboratory adopts a modular digestion oven, which can digest 20 samples at a time, the digestion temperature is easy to control, and the cycle is short. The automatic Kjeldahl nitrogen analyzer has a high-precision color sensor, can accurately determine the end point, and can automatically complete the distillation, titration, calculation , liquid and cleaning procedures, a sample measurement takes only 5min, reducing the random error, the data is accurate and reliable. Determination of crude protein content in crops and foods using the automatic Kjeldahl nitrogen analyzer can greatly improve the detection efficiency, accuracy and precision.
Select a representative seed to pick clean and shell, sample and grind, pass 0.42mm sieve. Weigh 0.5g sample 2 copies, accurate to 0.0001g, according to the national standard GB5497-1985 determine the moisture content of the sample. Open the digester and preheat it so that the temperature reaches 420 °C for digestion. Place the test crop sample in a 250-mL digestive canal (be careful not to adhere to the wall to prevent incomplete digestion). Except for soybean crop plus 1 g sample, all other crops are 0.5-1.0 g. After adding the digestion tablets 5g, then add 12 ~ 15mL concentrated sulfuric acid, gently shaking the digestive tube, the sample was wet with sulfuric acid. The digestion tube was placed on a digester preheated to 420°C and the suction pump was turned on to a maximum. After 5 minutes, a small suction pump was turned off until the acid mist just filled the exhaust hood and a condensation ring was formed in the test tube. About 90 min sample digestion to a clear blue-green liquid, remove and cool to room temperature.
Put the digestive tube into the automatic Kjeldahl nitrogen analyzer, close the safety gate, set the list, and start loading the sample after registration. The instrument is automatically distilled, titrated, and the results calculated and cleaned. Instead of using 0.1g of sucrose as a blank sample, the volume of the spent acid standard solution must not exceed 0.2mL.
The laboratory adopts a modular digestion oven, which can digest 20 samples at a time, the digestion temperature is easy to control, and the cycle is short. The automatic Kjeldahl nitrogen analyzer has a high-precision color sensor, can accurately determine the end point, and can automatically complete the distillation, titration, calculation , liquid and cleaning procedures, a sample measurement takes only 5min, reducing the random error, the data is accurate and reliable. Determination of crude protein content in crops and foods using the automatic Kjeldahl nitrogen analyzer can greatly improve the detection efficiency, accuracy and precision.
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