Blueberry in the process of cultivation and seedling cultivation techniques for seedlings need to be studied and summarized, so as to better promote the development of blueberry planting and improve economic efficiency. The use of blueberry tissue culture in the tissue culture chamber is a more recommended method. How do you do it?
(1) Field Selection The growing season selects robust, semi-lignified shoots. It is best to plant seedlings of explants in pots in greenhouses, draw materials from March to March each year, (2) inoculate, and divide the materials appropriately, and sterilize them on clean benches with 0.1% mercury for 6 to 10 minutes. Rinse with sterile water 5 times and insert into the medium.
(3) Induction culture with modified WPM medium, temperature 20-30 °C, light 12h / day, 30 days after the emergence of new shoots.
(4) Subculture Subculture After the establishment of the aseptic culture, the subculture is performed every 40-50 days to meet the requirement of seedling quantity. Temperature 20--30°C, Illumination 2000-3000LX, 12-16h.
(5) The seedlings to be transplanted will be prepared by the hardening seedlings, placed under strong light, and gradually opened to adapt to external conditions. It usually takes 7-15 days.
(6) Transplanting Remove the seedling from the bottle, remove the base medium and insert it into the seedling plate in the greenhouse. Under normal circumstances, one month later will survive.
(7) Incubation in the tissue culture room was started from September to November. The young shoots in the bottle were bottled, cut into 5-10 cm sections, and used with 1000-2000 ppm IAA or rooting powder, inserted in the matrix. The air humidity is 90% and the temperature is controlled at 20-28°C. Supplement the light with a biological lamp for 16 hours to prevent short-day seedlings from sleeping. Advantages: Relative to the spring of 5-6 months, the temperature in the solar greenhouse in September-November is mild, easy to control, and the rooting rate can reach 90%. In the 30 days before cutting rooting, no fertilizer is applied. After rooting, diammonium is sprayed once a week at a concentration of 1-5%. Until March of the following year, fertilization is stopped. In mid-May, as the temperature rises, the greenhouse gradually extinguishes the wind, hardening the seedlings for about 20-30 days. At this point, the seedling height is about 20-30 cm, with 3-5 branches. In mid-June, the seedlings were transplanted into 12-15 cm diameter pods and bred in open fields. The nutrient substrate follows the original plan: peat: organic fertilizer = 1: 1 : 1 and sulfur powder is added at 2 kg/m 3 . In the fall, you can cultivate large seedlings and plant them.
(8) Calling young shoots for rooting If the young shoots to be propagated need to be transported in a distant place or cannot be cut temporarily, a tissue culture room can be used to suspend the rooting method. That is, a 10 cm x 10 cm x 5 cm plastic food box (transparent) for ice cream is used. Cut 1cm wide, 30cm long paper strips, draw 10cm/L of IBA solution and lay it on the tabletop, then place it on every young branch of 2～2.5cm, roll it into small rolls, and put it in a plastic box. , put 8 to 10 rolls per box, a total of 100 young branches, add 2 ~ 3ml of 10mg/L IBA solution at the bottom of the box, cover and keep the humidity. Use a common mulch film to grow the tube, hold it at both ends, and then put the box into a stack of 8 to 10 boxes per tube. Hang in the indoor light and you can take root. This method can be used to promote rooting when young shoots are propagated in vitro in winter without greenhouse conditions.
(1) Field Selection The growing season selects robust, semi-lignified shoots. It is best to plant seedlings of explants in pots in greenhouses, draw materials from March to March each year, (2) inoculate, and divide the materials appropriately, and sterilize them on clean benches with 0.1% mercury for 6 to 10 minutes. Rinse with sterile water 5 times and insert into the medium.
(3) Induction culture with modified WPM medium, temperature 20-30 °C, light 12h / day, 30 days after the emergence of new shoots.
(4) Subculture Subculture After the establishment of the aseptic culture, the subculture is performed every 40-50 days to meet the requirement of seedling quantity. Temperature 20--30°C, Illumination 2000-3000LX, 12-16h.
(5) The seedlings to be transplanted will be prepared by the hardening seedlings, placed under strong light, and gradually opened to adapt to external conditions. It usually takes 7-15 days.
(6) Transplanting Remove the seedling from the bottle, remove the base medium and insert it into the seedling plate in the greenhouse. Under normal circumstances, one month later will survive.
(7) Incubation in the tissue culture room was started from September to November. The young shoots in the bottle were bottled, cut into 5-10 cm sections, and used with 1000-2000 ppm IAA or rooting powder, inserted in the matrix. The air humidity is 90% and the temperature is controlled at 20-28°C. Supplement the light with a biological lamp for 16 hours to prevent short-day seedlings from sleeping. Advantages: Relative to the spring of 5-6 months, the temperature in the solar greenhouse in September-November is mild, easy to control, and the rooting rate can reach 90%. In the 30 days before cutting rooting, no fertilizer is applied. After rooting, diammonium is sprayed once a week at a concentration of 1-5%. Until March of the following year, fertilization is stopped. In mid-May, as the temperature rises, the greenhouse gradually extinguishes the wind, hardening the seedlings for about 20-30 days. At this point, the seedling height is about 20-30 cm, with 3-5 branches. In mid-June, the seedlings were transplanted into 12-15 cm diameter pods and bred in open fields. The nutrient substrate follows the original plan: peat: organic fertilizer = 1: 1 : 1 and sulfur powder is added at 2 kg/m 3 . In the fall, you can cultivate large seedlings and plant them.
(8) Calling young shoots for rooting If the young shoots to be propagated need to be transported in a distant place or cannot be cut temporarily, a tissue culture room can be used to suspend the rooting method. That is, a 10 cm x 10 cm x 5 cm plastic food box (transparent) for ice cream is used. Cut 1cm wide, 30cm long paper strips, draw 10cm/L of IBA solution and lay it on the tabletop, then place it on every young branch of 2～2.5cm, roll it into small rolls, and put it in a plastic box. , put 8 to 10 rolls per box, a total of 100 young branches, add 2 ~ 3ml of 10mg/L IBA solution at the bottom of the box, cover and keep the humidity. Use a common mulch film to grow the tube, hold it at both ends, and then put the box into a stack of 8 to 10 boxes per tube. Hang in the indoor light and you can take root. This method can be used to promote rooting when young shoots are propagated in vitro in winter without greenhouse conditions.
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